เครื่องคำนวณการเจือจาง – C1V1 = C2V2

วิธีใช้เครื่องคิดเลขนี้

1. ป้อนInitial Concentration (C1)
2. ป้อนInitial Volume (V1, mL)
3. ป้อนFinal Concentration (C2)
4. คลิกปุ่มคำนวณ
5. อ่านผลลัพธ์ที่แสดงด้านล่างเครื่องคิดเลข

The Dilution Formula: C1V1 = C2V2

The dilution equation C₁V₁ = C₂V₂ states that the amount of solute is conserved when a solution is diluted. C₁ = initial concentration, V₁ = initial volume, C₂ = final concentration, V₂ = final volume.

To dilute a 10 M stock solution to 2 M in 50 mL: V₁ = (2 × 50) ÷ 10 = 10 mL. Take 10 mL of the 10 M solution and add 40 mL of solvent to get 50 mL of 2 M solution.

Dilution in Laboratory Practice

Dilutions are fundamental in biochemistry, molecular biology, and analytical chemistry. Common scenarios: preparing working solutions from stock solutions, creating serial dilutions for assays, and adjusting antibiotic concentrations for microbiology experiments.

Serial dilutions (1:10 repeating) are used in microbiology to estimate bacterial counts and in pharmacology for dose-response curves.

Units and Concentration Types

Concentration can be expressed as: molarity (M = mol/L), percent (% w/v or % v/v), mg/mL, μg/mL, or ppb/ppm. The C1V1=C2V2 formula works with any consistent unit — just ensure C1 and C2 use the same units, and V1 and V2 use the same volume units.

For example: If C1=100 mg/mL and you want C2=5 mg/mL in 200 mL, then V1=(5×200)/100=10 mL. Add 10 mL stock to 190 mL solvent.